Identification of the Putative Proton Donor Residue of Lacto-N-biose Phosphorylase (EC 2.4.1.211)The nucleotide sequences reported here are available in the GenBank sequence database under accession nos.AB181927 (lnpA1) and AB262011 (lnpA2)
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چکیده
Two lacto-N-biose phosphorylase (LNBP) isozyme genes were cloned from Bifidobacterium bifidum JCM1254. Alignment of the amino acid sequences of LNBP and its homologs identified 24 completely conserved acidic amino acid residues. All single mutants of Bifidobacterium longum LNBP at residues other than D313N retained considerable activity, suggesting that Asp313 is the putative proton donor residue in LNBP.
منابع مشابه
Practical Preparation of Lacto-N-biose I, a Candidate for the Bifidus Factor in Human MilkThe nucleotide and deduced amino acid sequences reported here are available in the GenBank sequence database under accession nos.AB303573 (GalT), AB303838 (SP), and AB303839 (GalE)
A one-pot enzymatic reaction to produce lacto-Nbiose I (LNB), which is supposed to represent the bifidus factor in human milk oligosaccharides, was demonstrated. Approximately 500mM of LNB was generated in 10-liter of reaction mixture initially containing 660 mM of sucrose and 600mM of GlcNAc by the concurrent actions of four enzymes, sucrose phosphorylase, UDPglucose—hexose-1-phospate uridylyl...
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متن کاملNovel putative galactose operon involving lacto-N-biose phosphorylase in Bifidobacterium longum.
A lacto-N-biose phosphorylase (LNBP) was purified from the cell extract of Bifidobacterium bifidum. Its N-terminal and internal amino acid sequences were homologous with those of the hypothetical protein of Bifidobacterium longum NCC2705 encoded by the BL1641 gene. The homologous gene of the type strain B. longum JCM1217, lnpA, was expressed in Escherichia coli to confirm that it encoded LNBP. ...
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تاریخ انتشار 2007